Journal: Clinical and Vaccine Immunology : CVI

Article Title: Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice

doi: 10.1128/CVI.00667-13

Figure Lengend Snippet: Specific antibody responses in mice elicited through immunization with P. acnes. BALB/c mice were randomly assigned to seven groups (n = 15 per group). Mice in groups 2 to 7 were immunized subcutaneously (s.c.) with viable P. acnes 14 (2 × 107 CFU, 2 × 108 CFU, and 2 × 109 CFU) and inactivated P. acnes 14 (2 × 107 CFU, 2 × 108 CFU, and 2 × 109 CFU). The control group (group 1) received 0.2 ml PBS only. One week later, all mice received the same dose of the respective immunogens. Thirty-five days after the first immunization, blood was drawn from the tail to collect serum for enzyme-linked immunosorbent assays (ELISAs). (A) Serum anti-P. acnes antibody titers. (B) Serum anti-A. pleuropneumoniae antibody titers. (C) Geometric mean of anti-P. acnes titers and anti-A. pleuropneumoniae serotype 1 titers. *, P < 0.05; **, P < 0.01; #, P > 0.05.

Article Snippet: However, there were no significant differences in the concentrations of IgM between the groups with different IgG titers. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Group Immunogen Anti- P. acnes IgG titer Mean concn (μg/ml) IgA IgM 1 PBS 0 139.142 ± 2.558 6.434 ± 0.150 2 Astragalus polysaccharides 0 140.482 ± 1.258 6.276 ± 0.492 3 P. acnes plus astragalus polysaccharides <100 at 35 days 141.887 ± 4.104 6.205 ± 0.260 4 P. acnes plus astragalus polysaccharides 400 135.798 ± 0.547 5.761 ± 0.126 5 P. acnes plus astragalus polysaccharides 800 139.091 ± 1.531 5.671 ± 0.233 6 P. acnes plus astragalus polysaccharides 1,600 142.240 ± 2.912 6.391 ± 0.458 7 P. acnes plus astragalus polysaccharides 3,200 146.418 ± 2.900 a 6.350 ± 0.138 8 P. acnes plus astragalus polysaccharides 6,400 146.597 ± 0.785 a 6.338 ± 0.443 Open in a separate window a The concentrations of IgA in groups 7 and 8 were significantly higher than those in the two control groups ( P < 0.01).

Techniques: Control

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice

doi: 10.1128/CVI.00667-13

Figure Lengend Snippet: Dynamic relationship between level of anti-P. acnes antibody and survival rates. Based on the different IgG titers at the indicated time points, all mice immunized with P. acnes with astragalus polysaccharide adjuvant were divided into eight groups (group 1, PBS control group, solid triangles; group 2, astragalus polysaccharide control group, solid circles; group 3, IgG titer of <100 at day 35, crosses; group 4, IgG titer of 400, open triangles; group 5, IgG titer of 800, open circles; group 6, IgG titer of 1,600, squares; group 7, IgG titer of 3,200, diamonds; group 8, IgG titer of 6,400, stars). Selected mice in each group were challenged with A. pleuropneumoniae serotype 1 (5× LD50). The survival rates of all groups were calculated at day 7 postchallenge. There were no significant differences among groups 3 to 8 (P > 0.05). Also, the survival rates of groups 3 to 8 were significantly higher than those of control groups 1 and 2. *, P < 0.05; **, P < 0.01; #, P > 0.05.

Article Snippet: However, there were no significant differences in the concentrations of IgM between the groups with different IgG titers. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Group Immunogen Anti- P. acnes IgG titer Mean concn (μg/ml) IgA IgM 1 PBS 0 139.142 ± 2.558 6.434 ± 0.150 2 Astragalus polysaccharides 0 140.482 ± 1.258 6.276 ± 0.492 3 P. acnes plus astragalus polysaccharides <100 at 35 days 141.887 ± 4.104 6.205 ± 0.260 4 P. acnes plus astragalus polysaccharides 400 135.798 ± 0.547 5.761 ± 0.126 5 P. acnes plus astragalus polysaccharides 800 139.091 ± 1.531 5.671 ± 0.233 6 P. acnes plus astragalus polysaccharides 1,600 142.240 ± 2.912 6.391 ± 0.458 7 P. acnes plus astragalus polysaccharides 3,200 146.418 ± 2.900 a 6.350 ± 0.138 8 P. acnes plus astragalus polysaccharides 6,400 146.597 ± 0.785 a 6.338 ± 0.443 Open in a separate window a The concentrations of IgA in groups 7 and 8 were significantly higher than those in the two control groups ( P < 0.01).

Techniques: Adjuvant, Control

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice

doi: 10.1128/CVI.00667-13

Figure Lengend Snippet: Indirect immunofluorescence. Rabbit anti-P. acnes hyperimmune serum was prepared (titer, ≈1:40,000). No immunofluorescence was detected in the absence of anti-P. acnes hyperimmune serum (A and B). Positive indirect immunofluorescence signals indicated binding of anti-P. acnes hyperimmune serum with A. pleuropneumoniae serotype 1 (C). This was consistent with the positive control (D).

Article Snippet: However, there were no significant differences in the concentrations of IgM between the groups with different IgG titers. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Group Immunogen Anti- P. acnes IgG titer Mean concn (μg/ml) IgA IgM 1 PBS 0 139.142 ± 2.558 6.434 ± 0.150 2 Astragalus polysaccharides 0 140.482 ± 1.258 6.276 ± 0.492 3 P. acnes plus astragalus polysaccharides <100 at 35 days 141.887 ± 4.104 6.205 ± 0.260 4 P. acnes plus astragalus polysaccharides 400 135.798 ± 0.547 5.761 ± 0.126 5 P. acnes plus astragalus polysaccharides 800 139.091 ± 1.531 5.671 ± 0.233 6 P. acnes plus astragalus polysaccharides 1,600 142.240 ± 2.912 6.391 ± 0.458 7 P. acnes plus astragalus polysaccharides 3,200 146.418 ± 2.900 a 6.350 ± 0.138 8 P. acnes plus astragalus polysaccharides 6,400 146.597 ± 0.785 a 6.338 ± 0.443 Open in a separate window a The concentrations of IgA in groups 7 and 8 were significantly higher than those in the two control groups ( P < 0.01).

Techniques: Immunofluorescence, Binding Assay, Positive Control

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice

doi: 10.1128/CVI.00667-13

Figure Lengend Snippet: Opsonization with anti-P. acnes serum enhances the phagocytosis of A. pleuropneumoniae by J774 murine macrophages. Opsonization with P. acnes hyperimmune rabbit serum increased the efficiency of uptake of A. pleuropneumoniae by J774 macrophages compared to control serum. In contrast, the fold intracellular CFU of group anti-P. acnes hyperimmune rabbit serum (10%) was significantly more than that of negative-control groups, including the preimmune rabbit serum, PBS, and unrelated (GST) hyperimmune rabbit serum groups (P < 0.01). Also, there were no significant differences in the uptake of A. pleuropneumoniae following opsonization with either P. acnes hyperimmune rabbit serum or A. pleuropneumoniae hyperimmune rabbit serum. Results are expressed as fold CFU of intracellular bacteria over the PBS treatment and are representative of three independent experiments performed in triplicate. Bars represent means ± standard deviations. Statistical analysis was carried out using the unpaired two-tailed Student t test. **, P < 0.01; #, P > 0.05.

Article Snippet: However, there were no significant differences in the concentrations of IgM between the groups with different IgG titers. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Group Immunogen Anti- P. acnes IgG titer Mean concn (μg/ml) IgA IgM 1 PBS 0 139.142 ± 2.558 6.434 ± 0.150 2 Astragalus polysaccharides 0 140.482 ± 1.258 6.276 ± 0.492 3 P. acnes plus astragalus polysaccharides <100 at 35 days 141.887 ± 4.104 6.205 ± 0.260 4 P. acnes plus astragalus polysaccharides 400 135.798 ± 0.547 5.761 ± 0.126 5 P. acnes plus astragalus polysaccharides 800 139.091 ± 1.531 5.671 ± 0.233 6 P. acnes plus astragalus polysaccharides 1,600 142.240 ± 2.912 6.391 ± 0.458 7 P. acnes plus astragalus polysaccharides 3,200 146.418 ± 2.900 a 6.350 ± 0.138 8 P. acnes plus astragalus polysaccharides 6,400 146.597 ± 0.785 a 6.338 ± 0.443 Open in a separate window a The concentrations of IgA in groups 7 and 8 were significantly higher than those in the two control groups ( P < 0.01).

Techniques: Control, Negative Control, Bacteria, Two Tailed Test

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Specific Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in Mice

doi: 10.1128/CVI.00667-13

Figure Lengend Snippet: Antibody responses in B cell depletion mice elicited through immunization with P. acnes. Mice in one B cell depletion model group and one normal control group (n = 10 per group) were immunized s.c. with viable P. acnes (2 × 108 CFU) on two occasions at 1-week intervals. Mice in the other B cell depletion model group were immunized with PBS. At day 35 after the first immunization, blood was drawn from the tail to collect serum for ELISAs. Immune serum reactivity was compared with that of preimmune serum of normal mice. The anti-P. acnes serum titers of B cell depletion mice vaccinated with P. acnes were significantly lower than those of normal mice vaccinated with P. acnes (P < 0.01). **, P < 0.01.

Article Snippet: However, there were no significant differences in the concentrations of IgM between the groups with different IgG titers. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Group Immunogen Anti- P. acnes IgG titer Mean concn (μg/ml) IgA IgM 1 PBS 0 139.142 ± 2.558 6.434 ± 0.150 2 Astragalus polysaccharides 0 140.482 ± 1.258 6.276 ± 0.492 3 P. acnes plus astragalus polysaccharides <100 at 35 days 141.887 ± 4.104 6.205 ± 0.260 4 P. acnes plus astragalus polysaccharides 400 135.798 ± 0.547 5.761 ± 0.126 5 P. acnes plus astragalus polysaccharides 800 139.091 ± 1.531 5.671 ± 0.233 6 P. acnes plus astragalus polysaccharides 1,600 142.240 ± 2.912 6.391 ± 0.458 7 P. acnes plus astragalus polysaccharides 3,200 146.418 ± 2.900 a 6.350 ± 0.138 8 P. acnes plus astragalus polysaccharides 6,400 146.597 ± 0.785 a 6.338 ± 0.443 Open in a separate window a The concentrations of IgA in groups 7 and 8 were significantly higher than those in the two control groups ( P < 0.01).

Techniques: Control